EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.28 | 0.0000025 | - |
3,N4-ethenocytosine-mismatched double-stranded DNA | - |
Escherichia coli | |
3.2.2.28 | 0.0000227 | - |
uracil-mismatched double-stranded DNA | - |
Escherichia coli | |
3.2.2.29 | 0.000012 | - |
uracil-mismatched double-stranded DNA | excision of uracil from U/G mismatches | Homo sapiens | |
3.2.2.29 | 0.0000128 | - |
thymine-mismatched double-stranded DNA | excision of thymine from T/G mismatches | Homo sapiens | |
3.2.2.29 | 0.0000243 | - |
3,N4-ethenocytosine-mismatched double-stranded DNA | excision of 3,N4-ethenocytosine from 3,N4-ethenocytidine/G mismatches | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.29 | 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O | Homo sapiens | 3,N4-ethenocytosine is recognized and efficiently excised by hTDG. The enzyme may be responsible for the repair of this mutagenic lesion in vivo and be important contributors to genetic stability | 3,N4-ethenocytosine + double-stranded DNA with abasic site | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.2.28 | Escherichia coli | - |
- |
- |
3.2.2.29 | Homo sapiens | Q13569 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.28 | 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O | 3,N4-ethenocytosine is recognized and efficiently excised from the 3,N4-ethenocytosine/G mismatch | Escherichia coli | 3,N4-ethenocytosine + double-stranded DNA with abasic site | - |
? | |
3.2.2.28 | additional information | the excision of thymine in a G/T mismatch by dsUDG is extremely low. This enzymatic activity does not have a real biological significance | Escherichia coli | ? | - |
? | |
3.2.2.28 | uracil-mismatched double-stranded DNA + H2O | excision of uracil from U/G mismatch | Escherichia coli | uracil + double-stranded DNA with abasic site | - |
? | |
3.2.2.29 | 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O | 3,N4-ethenocytosine is recognized and efficiently excised by hTDG. The enzyme may be responsible for the repair of this mutagenic lesion in vivo and be important contributors to genetic stability | Homo sapiens | 3,N4-ethenocytosine + double-stranded DNA with abasic site | - |
? | |
3.2.2.29 | 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O | 3,N4-ethenocytosine is recognized and efficiently excised from the 3,N4-ethenocytosine/G duplex oligonucleotide, when this residue is situated opposite to G. 26.5% of the activity measured with 3,N4-ethenocytosine mismatches is observed with 3,N4-ethenocytosine/A mismatches, 71% with 3,N4-ethenocytosine/A mismatches | Homo sapiens | 3,N4-ethenocytosine + double-stranded DNA with abasic site | - |
? | |
3.2.2.29 | thymine-mismatched double-stranded DNA + H2O | excision of thymine from T/G mismatches | Homo sapiens | thymine + double-stranded DNA with abasic site | - |
? | |
3.2.2.29 | uracil-mismatched double-stranded DNA + H2O | excision of uracil from U/G mismatches | Homo sapiens | uracil + double-stranded DNA with abasic site | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.2.28 | double-stranded uracil-DNA glycosylase | - |
Escherichia coli |
3.2.2.28 | dsUDG | - |
Escherichia coli |
3.2.2.29 | hTDG | - |
Homo sapiens |
3.2.2.29 | mismatch-specific thymine-DNA glycosylase | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.28 | 0.0028 | - |
uracil-mismatched double-stranded DNA | - |
Escherichia coli | |
3.2.2.28 | 0.016 | - |
3,N4-ethenocytosine-mismatched double-stranded DNA | - |
Escherichia coli | |
3.2.2.29 | 0.000015 | - |
thymine-mismatched double-stranded DNA | excision of thymine from T/G mismatches | Homo sapiens | |
3.2.2.29 | 0.000153 | - |
3,N4-ethenocytosine-mismatched double-stranded DNA | excision of 3,N4-ethenocytosine from 3,N4-ethenocytidine/G mismatches | Homo sapiens | |
3.2.2.29 | 0.00035 | - |
uracil-mismatched double-stranded DNA | excision of uracil from U/G mismatches | Homo sapiens |